Supplementary Material 1 2 Methods 3 4 5 6 7 8 9 10 11 12 13 14 15

supplementary material 1 2 methods 3 4 5 6 7 8 9 10 11 12 13 14 15

Cell culture and transfection. The human lung cancer cell H1299, human embryonic kidney HEK 293T and mouse embryonic fibroblast NIH3T3 were obtained from and maintained as instructed by the ATCC. All transfections were carried out using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. To ectopically express hNaa10p or DNMT1, cells were seeded 1 day before transfection of the plasmid encoding indicated protein and harvested 24 h post transfection. To knock down hNaa10p or DNMT1, 2x106 cells were seeded in 15-cm dish 1 day before transfection of 10 nM corresponding siRNA using Lipofectamine 2000 every 24 hours for three times.